Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site

dc.contributor.authorRachkevych, Nazarii
dc.contributor.authorSybirna, Kateryna
dc.contributor.authorBoyko, Solomiya
dc.contributor.authorBoretsky, Yuriy
dc.contributor.authorSibirny, Andriy
dc.contributor.authorБорецький, Юрій
dc.date.accessioned2018-05-15T17:13:30Z
dc.date.available2018-05-15T17:13:30Z
dc.date.issued2014
dc.description.abstractHere we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensisMR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used.uk_UA
dc.identifier.citationImproving the efficiency of plasmid transformation in Shewanella oneidensis MR - 1 by removing ClaI restriction site / Rachkevych N., Sybirna K., Boyko S., Boretsky Y., Sibirny A. // Journal of Microbiological Methods. – 2014 . – Vol. 99 . – P. 35 – 37. (Scopus)uk_UA
dc.identifier.urihttp://repository.ldufk.edu.ua/handle/34606048/10460
dc.language.isoenuk_UA
dc.subjectDNA restriction–modificationuk_UA
dc.subjectShewanella oneidensisuk_UA
dc.subjectTransformationuk_UA
dc.titleImproving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction siteuk_UA
dc.typeArticleuk_UA

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