Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site
dc.contributor.author | Rachkevych, Nazarii | |
dc.contributor.author | Sybirna, Kateryna | |
dc.contributor.author | Boyko, Solomiya | |
dc.contributor.author | Boretsky, Yuriy | |
dc.contributor.author | Sibirny, Andriy | |
dc.contributor.author | Борецький, Юрій | |
dc.date.accessioned | 2018-05-15T17:13:30Z | |
dc.date.available | 2018-05-15T17:13:30Z | |
dc.date.issued | 2014 | |
dc.description.abstract | Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensisMR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used. | uk_UA |
dc.identifier.citation | Improving the efficiency of plasmid transformation in Shewanella oneidensis MR - 1 by removing ClaI restriction site / Rachkevych N., Sybirna K., Boyko S., Boretsky Y., Sibirny A. // Journal of Microbiological Methods. – 2014 . – Vol. 99 . – P. 35 – 37. (Scopus) | uk_UA |
dc.identifier.uri | http://repository.ldufk.edu.ua/handle/34606048/10460 | |
dc.language.iso | en | uk_UA |
dc.subject | DNA restriction–modification | uk_UA |
dc.subject | Shewanella oneidensis | uk_UA |
dc.subject | Transformation | uk_UA |
dc.title | Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site | uk_UA |
dc.type | Article | uk_UA |
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