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  • Документ
    Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site
    (2014) Rachkevych, Nazarii; Sybirna, Kateryna; Boyko, Solomiya; Boretsky, Yuriy; Sibirny, Andriy; Борецький, Юрій
    Here we demonstrate that elimination of ClaI restriction site from the sequence of a plasmid DNA increases the efficiency of transformation of Shewanella oneidensis MR-1 significantly. To achieve reliable transformation of S. oneidensisMR-1 plasmids either lacking ClaI site or isolated from primary transformants of S. oneidensis should be used.