Please use this identifier to cite or link to this item: https://repository.ldufk.edu.ua/handle/34606048/4404
Title: Molecular Cloning of the GTP - Cyclohydrolase Structural Gene RIB1 of Pichia guilliermondii involved in riboflavin biosynthesis
Authors: Liauta - Teglivets, O.
Hasslache, M.
Boretsky, Yuriy
Kohlwein, S.
Shavlovskii, G.
Keywords: yeast
riboflavin
GTP-cyclohydrolase
DNA sequence
Issue Date: 1995
Citation: Molecular Cloning of the GTP - Cyclohydrolase Structural Gene RIB1 of Pichia guilliermondii involved in riboflavin biosynthesis / Liauta - Teglive ts O., Hasslacher M., Boretskii Y. R., Kohlwein S. D., Shavlovskii G. M. // Yeast. – 1995. – Vol. 11. – P. 945 – 952.
Abstract: The structural gene of GTP-cyclohydrolase, involved in riboflavin biosynthesis, was cloned from a Pichia guilliermondii genomic library. A 1855 bp genomic DNA fragment complementing the riboflavin auxotrophies of an Escherichia coli ribA mutant, defective in GTP-cyclohydrolase 11, and a P. guilliermondii rib1 mutant was isolated and sequenced. An open reading frame with the potential to encode a protein of 344 amino acids with a predicted molecular mass of 38 71 1 Da was detected. The P. guilliermondii enzyme shows a high degree of homology to GTP-cyclohydrolases type I1 from E. coli and Baccillus subtilis and to GTP-cyclohydrolase from Saccharomyces cerevisiue. Functional GTP-cyclohydrolase from P. guilliermondii may consist of four identical subunits. The sequence of the RlBl gene of P. guilliermondii was submitted to the EMBL sequence database and is accessible under Accession Number 249093.
URI: http://repository.ldufk.edu.ua/handle/34606048/4404
Appears in Collections:Наукові праці професорсько-викладацького складу ЛДУФК

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