Restoration of the Wild-Type Phenotype in Pichia guilliermondii Transformants

Please use this identifier to cite or link to this item: https://repository.ldufk.edu.ua/handle/34606048/10683

Title:	Restoration of the Wild-Type Phenotype in Pichia guilliermondii Transformants
Authors:	Pinyaga, Yu. Prokopiv, T. Petrishin, A. Khalimonchuk, O. Protchenko, O. Fedorovich, D. Boretsky, Yuriy Борецький, Юрій
Keywords:	Yeast Pichia guilliermondii transformation recombination types riboflavin biosynthesis GTP cyclohydrolase II
Issue Date:	2002
Citation:	Restoration of the Wild-Type Phenotype in Pichia guilliermondii Transformants / Yu. V. Pinyaga, T. M. Prokopiv, A. V. Petrishin, O. V. Khalimonchuk, O. V. Protchenko, D. V. Fedorovich, Yu. R. Boretsky // Microbiology. - 2002. - Vol. 71, No. 3. - P. 368–372.
Abstract:	Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.
URI:	http://repository.ldufk.edu.ua/handle/34606048/10683
Appears in Collections:	Наукові праці професорсько-викладацького складу ЛДУФК в базах даних Scopus, WoS, Tomson Reuters

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