Please use this identifier to cite or link to this item: http://repository.ldufk.edu.ua/handle/34606048/10398
Title: Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii
Authors: Boretsky, Yuriy
Voronovsky, Andriy
Liuta-Tehlivets, Oksana
Hasslacher, Meinhard
Kohlwein, Sepp D.
Shavlovsky, Georgiy M.
Борецький, Юрій
Keywords: Autonomously replicating sequence
Flavinogenic yeast
GTP cyclohydrolase II
Transformation system
Issue Date: 1999
Citation: Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii / Boretsky Y ., Voronovsky A ., Liuta - Tehlivets O ., Hasslacher M ., Kohlwein S . D ., Shavlovsky G . M. // Curr Genet . – 1999. – Vol. 36, N 4. – P. 215 – 221. (Scopus)
Abstract: An Autonomously Replicating Sequence ele- ment adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identi®ed by transformation experiments. Detailed se- quence analysis unveiled two potential ARS elements located 5¢ and 3¢ of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like se- quence 3¢ to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 104±105 transformants/lg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for e cient transformation of the ¯avinogenic yeast P. guilliermondii was developed.
URI: http://repository.ldufk.edu.ua/handle/34606048/10398
Appears in Collections:Наукові праці професорсько-викладацького складу ЛДУФК в базах даних Scopus, WoS, Tomson Reuters

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