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https://repository.ldufk.edu.ua/handle/34606048/10406
Назва: | Positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast Pichia guilliermondii |
Автори: | Boretsky, Yuriy Kapustyak, Kostyantyn Fayura, Lyubov Stasyk, Oleh Stenchuk, Mykola Bobak, Yaroslav Drobot, Lyudmyla Sibirny, Andriy Борецький, Юрій |
Ключові слова: | Yeast Pichia guilliermondii Riboflavin biosynthesis regulation GTP cyclohydrolase II Mutability Iron |
Дата публікації: | 2005 |
Бібліографічний опис: | Positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast Pichia guilliermondii / Boretsky Y. R., Kapustyak K. Y., Fayura L. R., Stasyk O. V., Stenchuk M. M., Bobak Y. P., Drobot L. B., Sibirny A. A. // FEMS Yeast Res. – 2005. – Vol. 5(9). – P. 829 – 837. (Scopus) |
Короткий огляд (реферат): | It is known for many years that iron represses synthesis of riboflavin (RF) and most of RF-synthesizing enzymes in several yeast species, known as flavinogenic yeasts. However, the mechanism of such repression is not known. We have found that iron represses transcription of RIB1 and RIB7 genes coding for the first and the last enzymes of RF biosynthesis in the model flavinogenic organism Pichia guilliermondii. To decipher molecular mechanisms of iron-dependent repression, isolation and study of the regulatory mutants defective in corresponding regulation is desirable. However, no suitable methods for isolation of such mutants were previously available. We have produced a single-point transition mutation in the RIB1 gene. The corresponding rib1-86 mutant exhibits leaky phenotype and is unable to grow in iron-sufficient minimal medium without exogenous RF. However, it can grow in minimal iron-deficient medium without RF, or in iron-sufficient medium upon introduction of the previously-isolated regulatory mutation rib81, which leads to increase in RF production. Using the rib1-86 mutant as parental strain, a collection of mutants able to grow in iron-sufficient medium without exogenous RF has been isolated. The mutants appeared to be defective in regulation of RF biosynthesis and iron homeostasis and were divided into six new complementation groups. Study of one corresponding mutant, red6, showed derepression of RIB1 mRNA synthesis in iron-sufficient medium. |
URI (Уніфікований ідентифікатор ресурсу): | http://repository.ldufk.edu.ua/handle/34606048/10406 |
Розташовується у зібраннях: | Наукові праці професорсько-викладацького складу ЛДУФК в базах даних Scopus, WoS, Tomson Reuters |
Файли цього матеріалу:
Файл | Розмір | Формат | |
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Boreckirib186_Poz_sel_bor_FEMS_05.pdf | 417.26 kB | Adobe PDF | Переглянути/Відкрити |
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